Route of Injection Affects the Impact of InlB Internalin Domain Variants on Severity of Listeria monocytogenes Infection in Mice

Figure 1

Impact of idInlB variants on severity of intravenous infection in BALB/c mice. (a) Mortality caused by parental EGDeΔinlB, wild-type EGDe, and recombinant EGDeΔinlB::InlB1 (idInlB1), EGDeΔinlB::InlB9 (idInlB9), and EGDeΔinlB::InlB14 (idInlB14) strains. Groups of 20 mice were infected i.v. with 2 × 10⁶ CFU as described in Materials and Methods. (b) Proliferation of EGDeΔinlB::InlB14 in the liver and spleen of mice i.v. infected with 1 × 10⁴ CFU. Average and SD for groups of 5 mice are shown. (c) and (d) idInlB-dependent drop of bacterial loads in the liver and spleen 6 (c) and 24 (d) hpi. BALB/c mice were i.v. infected with 1 × 10⁶ CFU. The data and average of two independent experiments performed on groups of 5 mice are shown.

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