BioMed Research International / 2017 / Article / Tab 1 / Research Article
Route of Injection Affects the Impact of InlB Internalin Domain Variants on Severity of Listeria monocytogenes Infection in Mice Table 1 Strains and plasmids used in the work.
Species/strain Characteristics Reference L. monocytogenes Serovar 1/2a, type strain [27 ] EGDe derivative with inlB gene deletion [28 ] VIMHA004 Serovar 4b, , clinical isolate obtained from the stillborn, carried [21 ] VIMHA015 Serovar 4b, , Clinical isolate obtained from the stillborn, carried [21 ] VIMHA034 Serovar 1/2a, , Clinical isolate obtained from the stillborn, carried [21 ] EGDeΔinlB::InlB1 EGDeΔinlB supplemented with the pInlB1 plasmid [25 ] EGDeΔinlB::InlB9 EGDeΔinlB supplemented with the pInlB9 plasmid [25 ] EGDeΔinlB::InlB14 EGDeΔinlB supplemented with the pInlB14 plasmid [25 ] E. coli JM109 endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+Δ(lac-proAB) e14-[F′ traD36 proAB+lacIqlacZΔM15] hsdR17(rK-mK+) Promega BL21 (DE3) F–ompT gal dcm lon hsdSB(rB-mB-) λ (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) NewEngland BioLabs Plasmids pTgem-easy Cloning vector Promega pTRKH2 Shuttle-vector [29 ] pInlAB pTRKH2:: : :gwdI [25 ] pInlB1 pInlAB::idInlB1 [25 ] pInlB9 pInlAB::idInlB9 [25 ] pInlB14 pInlAB::idInlB14 [25 ]
strain was generously provided by Professor J. A. Vazquez-Boland, University of Edinburgh. tyes (STs) are provided according to the MLST protocol by Ragon et al., 2008. is an internalin domain of InlB. 4 gwdInlB is InlB GW-domain.