Route of Injection Affects the Impact of InlB Internalin Domain Variants on Severity of Listeria monocytogenes Infection in Mice

Table 1

Strains and plasmids used in the work.

Species/strain	Characteristics	Reference
L. monocytogenes
	Serovar 1/2a, type strain	[27]
	EGDe derivative with inlB gene deletion	[28]
VIMHA004	Serovar 4b, , clinical isolate obtained from the stillborn, carried	[21]
VIMHA015	Serovar 4b, , Clinical isolate obtained from the stillborn, carried	[21]
VIMHA034	Serovar 1/2a, , Clinical isolate obtained from the stillborn, carried	[21]
EGDeΔinlB::InlB1	EGDeΔinlB supplemented with the pInlB1 plasmid	[25]
EGDeΔinlB::InlB9	EGDeΔinlB supplemented with the pInlB9 plasmid	[25]
EGDeΔinlB::InlB14	EGDeΔinlB supplemented with the pInlB14 plasmid	[25]
E. coli
JM109	endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+Δ(lac-proAB) e14-[F′ traD36 proAB+lacIqlacZΔM15] hsdR17(rK-mK+)	Promega
BL21 (DE3)	F–ompT gal dcm lon hsdSB(rB-mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])	NewEngland BioLabs
Plasmids
pTgem-easy	Cloning vector	Promega
pTRKH2	Shuttle-vector	[29]
pInlAB	pTRKH2::::gwdI	[25]
pInlB1	pInlAB::idInlB1	[25]
pInlB9	pInlAB::idInlB9	[25]
pInlB14	pInlAB::idInlB14	[25]

strain was generously provided by Professor J. A. Vazquez-Boland, University of Edinburgh. tyes (STs) are provided according to the MLST protocol by Ragon et al., 2008. is an internalin domain of InlB. ⁴gwdInlB is InlB GW-domain.