Immune Response and Protective Efficacy of a Heterologous DNA-Protein Immunization with Leishmania Superoxide Dismutase B1

Figure 2

Antigen-specific antibody response in BALB/c mice before challenge. Mice were immunized twice with LdFeSODB1 DNA antigens and controls followed by a boost with the recombinant LdFeSODB1 protein. All immunizations were given in three-week intervals. Blood samples were collected before immunization, at the time of each immunization and upon euthanasia. Total IgG (a), IgG1 (b), and IgG2a (c) against rLdFeSODB1 were measured using ELISA and the result is depicted as mean of five mice per group and standard error of the mean (SEM). The mean IgG2a/IgG1 ratio is depicted in (d). Statistical comparison between groups was performed using Mann–Whitney test. The assay was done in duplicate wells for each mouse serum. This is one of two experiments with similar result. The asterisk shows statistically significant difference between the vaccine antigen and the respective control groups ().

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