Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators

Figure 2

Effect of small molecules on MK polyploidization in human MK progenitors (MK-PROs). (a) MK-PROs DNA ploidy analysis (4N and ≥8N) after different small molecules treatment. Representative flow cytometry plots are shown in the upper panel. Statistical analysis was made by comparing with the control group. (b) Morphology and cell diameter of MK-PROs after small molecules treatment for 10 days (scale bars: 50 μm). (c) The proportion of CD61⁺ cells after treated with four small molecules was normalized to control. (d) The apoptosis analysis by flow cytometry in MK-PROs. Histogram of the proportion of early apoptosis and late apoptosis in MK-PROs treated with or without small molecules is showed in the lower panel. (e) The cell proliferation with or without small molecules. Results are means and SEM from biological replicates (). ; ; .

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