Small Molecule Supplements Improve Cultured Megakaryocyte Polyploidization by Modulating Multiple Cell Cycle Regulators

Figure 3

The combination of small molecules for polyploidization in MKs. (a) Ploidy analysis by flow cytometry. Ploidy (≥8N) after the combination of small molecule treatment was compared with control in three megakaryocytic cells. Representative flow cytometry plots of RRI + SI treatment or control are shown in the lower panel. (b) Morphology of stained polyploid megakaryocytes in UT-7 cells treated with RRI and SI (scale bars: 20 μm). (c) Cell diameter of the MK-PROs after small molecule combinations treatment for 10 days. (d) The proportion of CD61⁺ cells from MK-PROs treated with the combined small molecules, normalized to control. (e) The early apoptosis and late apoptosis were detected by flow cytometry. Lower panel: the proportion of apoptosis in MK-PROs treated with combined small molecules, compared with control. (f) Ploidy (4N and ≥8N) in MK-PROs treated with RRI and SI was compared with control. Results are means and SEM from biological replicates (). ; ; .

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