Automated Image Analysis of HER2 Fluorescence In Situ Hybridization to Refine Definitions of Genetic Heterogeneity in Breast Cancer Tissue

Figure 1

Distribution of tumors with regard to genetic heterogeneity and amplification guidelines. (a) Percentage of amplified cells plotted for MP and AD values, Amp_Cell_%_M and Amp_Cell_%_A, respectively. Dashed line marks identity line. AD overestimates low MP values (crosses) whereas it underestimates MP values in the range >28 (circles); (b) HER2_M plotted against Ratio_M with cut-offs for amplification by ASCO/CAP 2013 guidelines shown by grey lines. Cases marked with crosses are overestimated cases from (a); (c) Cell_Amp_%_M plotted against Ratio_M, horizontal lines at 5% and 50% mark cut-off values for determining GH cases. Note the lack of cases in the 3–28% range; (d) HER2_A plotted against RATIO_A, amplification cut-off marked in grey; (e) Amp_Cell_%_A plotted against RATIO_A. Summary: MP: 13 positive, 21 equivocal, 16 negative, and 8 GH cases; AD: 7 positive, 3 equivocal, 40 negative, and 36 GH cases.

(a)

(b)

(c)

(d)

(e)