Automated Image Analysis of HER2 Fluorescence In Situ Hybridization to Refine Definitions of Genetic Heterogeneity in Breast Cancer Tissue

Table 1

Regression analysis of HER2 copy number, CEP17 copy number, and HER2/CEP17 ratio. Automated data (AD) was tested as dependent variable to estimate its prediction from the corrected data (CD) and manual procedure (MP) results.

Variable		Intercept	Intercept	Slope	Slope
AD dependent, CD explanatory
HER2 copy number	0.989	0.209		0.918
CEP17 copy number	0.992	0.073		0.968
HER2/CEP17 ratio	0.985	0.099		0.915
AD dependent, MP explanatory
HER2 copy number	0.851	0.610		0.557
CEP17 copy number	0.84	0.464		0.621
HER2/CEP17 ratio	0.92	0.335		0.784