Automated Image Analysis of HER2 Fluorescence In Situ Hybridization to Refine Definitions of Genetic Heterogeneity in Breast Cancer Tissue

Table 2

Characteristics of the clusters extracted from the automated image analysis data. GH by Cell_Amp_%_M: percentage of amplified cells detected by manual procedure, calculated from HER2/CEP17 ratio and by HER2 signal only. AshD_Ratio, AshD_HER2, and AshD_CEP17: Ashman’s D indicator calculated for HER2/CEP17, HER2, and CEP17 automated data.

	Cluster 1	Cluster 2	Cluster 3	Cluster 4	Total
Number of observations	1	9	18	22	50
Amplification; (amp/equiv/neg)	1/0/0	9/0/0	0/4/14	3/17/2	50
Polysomy	0	0	3	19	22
GH by Cell_Amp_%_M; (by HER2/CEP17 ratio)	0	1	1	6	8
GH by Cell_Amp_%_M; (by HER2 only)	0	4	6	17	27
AshD_Ratio > 2	1	1	2	1	5
AshD_Her2 > 2	1	4	6	12	23
AshD_CEP17 > 2	1	0	5	5	11
Predominantly	Amplified	Amplified	Negative	Equivocal; Polysomic; Bimodal