Rapid Chondrocyte Isolation for Tissue Engineering Applications: The Effect of Enzyme Concentration and Temporal Exposure on the Matrix Forming Capacity of Nasal Derived Chondrocytes

Figure 2

(a) Cell yield (×10⁶ per g of cartilage) for incubation times of 1 h and 12 h with 750 or 3000 U/ml of collagenase with physical agitation . (b) Cell viability (%). (c) Population doubling time (days) with initial seeding density of 5 × 10³ cells/cm². to 1 h incubation for the same enzyme concentration and to 750 U/ml enzyme concentration for the same incubation period . (d) Evaluation of cell morphology with crystal violet (CV), hematoxylin and eosin (H&E), and fluorescent DAPI/F-Actin staining following 7 days of expansion (initial seeding density 5 × 10³ cells/cm²). Scale bar: 500 µm.

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