Expression of C4.4A in an In Vitro Human Tissue-Engineered Skin Model

Figure 4

C4.4A in tissue-engineered wound healing model. (a-b) Tissue section of a human TES stained for C4.4A (green in (a) and red in (b)) and the α3 subunit of integrin (green in (b)) two days after the creation of a wound. Cell nuclei are stained with Hoechst (blue). Note that the foremost suprabasal keratinocytes of the migrating front that highly express the α3 subunit of integrin are devoid of C4.4A (arrows). (a′) Phase contrast image corresponding to panel (a). α3, α3 subunit of integrin; AW, after wound; TES, tissue-engineered skin. Scale bar = 50 μm.