Abdominal Wall Reconstruction after Flap Surgery and the Effect on the Immune System

Table 1

Toll-like receptor genes role and primers sequence used for qRT-PCR analysis.

Symbol	Gene name	Cellular localization and role	Primer sequence
TLR1	Toll-like receptor 1	Localized on the cell surface.; Recognizing microbial components.	TGCTGGATGCTAACGGATGTC; AAGTGGTTTCAATGTTGTTCAAAGTC
TLR2	Toll-like receptor 2		TCACTTGTCTAACTTATCATCCTCTTG; TCAGCGAAGGTGTCATTATTGC
TLR4	Toll-like receptor 4		GCCATCGCTGCTAACATCATC; CTCATACTCAAAGATACACCATCGG
TLR6	Toll-like receptor 6		AACCTACTGTCATAAGCCTTCATTC; GTCTACCACAAATTCACTTTCTTCAG
TLR8	Toll-like receptor 8	Intracellular vesicles.; Recognizing nucleic acids.	AAGACCACCACCAACTTAGCC; GACCCTCAGATTCTCATCCATCC
TLR9	Toll-like receptor 9		CACGACAGCCGAATAGCAC; GGGAACAGGGAGCAGAGC
TLR10	Toll-like receptor 10	Surface of B cells, monocytes, and neutrophils.; Anti-inflammatory function.	CCTGTCCAACTGCCTCATTTG; CTAAGTGTTCTAAGGATGTGTTTCTG