Abdominal Wall Reconstruction after Flap Surgery and the Effect on the Immune System
Table 2
Cytokine genes role and primers sequence used for qRT-PCR analysis.
Symbol
Gene name
Role
Primer sequence
TNFα
Tumor necrosis factor
Implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
CAGCCTCTTCTCCTTCCTGAT TGGGGAACTCTTCCCTCTG
IL-1β
Interleukin 1β
Inflammatory response. Involved in cell proliferation, differentiation, and apoptosis.
CAGCCAATCTTCATTGCTCA GCATCTTCCTCAGCTTGTCC
IL-6
Interleukin 6
Involved in inflammation-associated disease.
GATGAGTACAAAAGTCCTGATCCA CTGCAGCCACTGGTTCTGT
IFNγ
Interferon Gamma
Mutations in this gene are associated with an increased susceptibility to viral, bacterial, and parasitic infections and to several autoimmune diseases.
TCATCCAAGTGATGGCTGAA ATATTGCAGGCAGGACAACC
β-Actin
Housekeeping genes
With a maintenance in basic cellular function role the housekeeping genes are expressed in all organisms’ cells.
