Extremely Low-Frequency Electromagnetic Fields Affect Myogenic Processes in C2C12 Myoblasts: Role of Gap-Junction-Mediated Intercellular Communication

Figure 5

Functional coupling in differentiating C2C12 myoblasts during ELF-EMF treatments. (a) Representative phase contrast and corresponding fluorescence images of the C2C12 myoblasts in the absence (Ctr) and presence of ELF-EMF treatment with 1.0 mT, from the beginning of the differentiation process (0, time 0) and after 24 h and 48 h in differentiation medium. Scale bars, 50 μm. (b) Quantification of dye-coupled cells under the conditions described in (a). Data are means ± SEM from two independent experiments, each performed with at least 10 independent microinjections/plate. versus corresponding Ctr.

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