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BioMed Research International
Volume 2017 (2017), Article ID 2472805, 6 pages
Research Article

Testicular Damage following Testicular Sperm Retrieval: A Ram Model Study

1Centre of Andrology, Odense University Hospital, Odense, Denmark
2Fertility Clinic, Odense University Hospital, Odense, Denmark
3Department of Pathology, Odense University Hospital, Odense, Denmark

Correspondence should be addressed to Jens Fedder

Received 19 May 2017; Accepted 22 August 2017; Published 27 September 2017

Academic Editor: Mohamed Elshazly

Copyright © 2017 Jens Fedder et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to evaluate the possible development of histological abnormalities such as fibrosis and microcalcifications after sperm retrieval in a ram model. Fourteen testicles in nine rams were exposed to open biopsy, multiple TESAs, or TESE, and the remaining four testicles were left unoperated on as controls. Three months after sperm retrieval, the testicles were removed, fixed, and cut into 1/2 cm thick slices and systematically put onto a glass plate exposing macroscopic abnormalities. Tissue from abnormal areas was cut into 3 μm sections and stained for histological evaluation. Pathological abnormalities were observed in testicles exposed to sperm retrieval (≥11 of 14) compared to 0 of 4 control testicles. Testicular damage was found independently of the kind of intervention used. Therefore, cryopreservation of excess sperm should be considered while retrieving sperm.