The Kinase STK3 Interacts with the Viral Structural Protein VP1 and Inhibits Foot-and-Mouth Disease Virus Replication

Figure 4

STK3 inhibits FMDV replication during virus infection. (a) PK-15 cells were seeded in 6-well plates, and the monolayer cells were transfected with 1, 2, and 3 μg of a Myc-STK3 expressing plasmid and 3 μg of an empty Myc vector. The empty Myc vector was used in the transfection process to ensure that the same amount of cells received the same amount of total plasmids. At 24 hpt, the cells were mock infected or infected with FMDV (MOI 0.5) for 12 h. The expression of viral RNA and the VP1 protein was detected by qPCR assay and western blotting, respectively. (b) PK-15 cells were seeded in 3.5 cm dish, and the monolayer cells were transfected with 150 nM of STK3 siRNA or NC siRNA for 48 h, followed by infection with equal amounts of FMDV (MOI 0.5). The cells were collected at the indicated time points (0, 6, and 12 h). Expression of STK3 mRNA and viral RNA was determined by qPCR assay. Expression of STK3 and the viral VP1 protein was detected by western blotting.

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