Mutagenic and Cytotoxicity LQB 123 Profile: Safety and Tripanocidal Effect of a Phenyl-t-Butylnitrone Derivative

Table 2

Number of Salmonella typhimurium TA97, TA198, TA100, TA1535, and TA102 revertant colonies per plate in the presence of the PBN derivate following the preincubation procedure of the Salmonella/microsome assay in the absence (−) and in the presence (+) of exogenous metabolizing system from rat liver S9-mix.

Strain	Dose (µM)	−S9		+S9
		Mean ± SD	MI	Mean ± SD	MI
TA97	0	112 ± 9	1.0	166 ± 19	1.0
	22	114 ± 20	1.0	192 ± 15	1.2
	220	92 ± 22	0.8	192 ± 18	1.2
	2200	97 ± 25	0.9	183 ± 11	1.1
	22000	11 ± 2	0.1	183 ± 18	1.1
	33000	1 ± 0	0.0	155 ± 11	0.9
	PC	1304 ± 226	12.0	1488 ± 139	9.0
TA98	0	24 ± 9	1.0	19 ± 1	1.0
	22	31 ± 2	1.3	22 ± 6	1.2
	220	29 ± 1	1.2	18 ± 7	1.0
	2200	25 ± 4	1.0	19 ± 1	1.0
	22000	8 ± 3	0.3	18 ± 9	1.0
	33000	7 ± 2	0.3	20 ± 2	1.1
	PC	613 ± 32	26.0	291 ± 36	16.0
TA100	0	201 ± 35	1.0	272 ± 45	1.0
	22	221 ± 28	1.1	242 ± 41	0.9
	220	205 ± 5	1.0	274 ± 35	1.0
	2200	154 ± 19	0.8	266 ± 38	1.0
	22000	134 ± 10	0.7	226 ± 33	0.8
	33000	52 ± 0	0.3	215 ± 25	0.8
	PC	637 ± 52	3.2	1257 ± 107	4.6
TA1535	0	13 ± 0	1.0	17 ± 3	1.0
	22	15 ± 2	1.2	17 ± 3	1.0
	220	14 ± 2	1.1	15 ± 3	0.9
	2200	11 ± 3	0.8	12 ± 1	0.7
	22000	11 ± 2	0.8	12 ± 1	0.7
	33000	12 ± 2	0.9	12 ± 1	0.7
	PC	615 ± 83	47.3	168 ± 18	10.1
TA102	0	433 ± 10	1.0	202 ± 12	1.0
	22	449 ± 30	1.0	214 ± 16	1.1
	220	427 ± 29	1.0	218 ± 8	1.1
	2200	409 ± 45	0.9	201 ± 11	1.0
	22000	534 ± 67	1.2	181 ± 26	0.9
	33000	533 ± 15	1.2	152 ± 7	0.8
	PC	1638 ± 201	3.8	1136 ± 78	5.6

Number of revertant colonies per plate: mean and standard deviation (SD) values of three replicates. MI: mutagenicity index: ratio of the number of revertant colonies induced by the samples/spontaneous number by the negative control. Positive mutagenicity (MI > 2) is indicated in bold. The sample vehicle (DMSO) was tested as a negative control. The doses of the positive controls (PC) in assays without S9: 526 μM and 263 μM of 4-NQO (TA97 and TA98, resp.), 769 μM and 154 μM of AS (TA100 and TA1535, resp.), and 149 μM of MMS (TA102). In assays with S9: 396 μM of B[α]P (TA102) and 517 μM of 2-AA (for others strains), statistically significant differences () relative to the negative control by ANOVA and Tukey’s test are indicated.