Dab1 Contributes to Angiotensin II-Induced Apoptosis via p38 Signaling Pathway in Podocytes

Figure 3

Ang II increases reelin/Dab1 expression in cultured podocytes and induces podocytes apoptosis. (a) Western blotting detected the expressions of phospho-Dab1, Dab1, and reelin in cultured podocytes treated by Ang II (10⁻⁶ M) at various time points. (b, c) Quantitative analysis of phospho-Dab1 and reelin expression. (d) Immunofluorescence staining of Dab1, phospho-Dab1, and reelin in cultured podocytes stimulated by Ang II (10⁻⁶ M) for 6 h (original magnification ×400); scale bars, 20 μm. (e) Western blotting detected the expressions of cleaved caspase-3 and bcl-2 in cultured podocytes treated by Ang II (10⁻⁶ M) at various time points. (f) Quantitative analysis of cleaved caspase-3 and bcl-2 expressions. versus control group. ^#P < 0.05 versus control group.

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