Effect of Wnt Signaling on the Differentiation of Islet β-Cells from Adipose-Derived Stem Cells

Table 2

Primers used for quantitative PCR analysis of islet β-cell growth and function genes.

Primer name	Primer sequence (5′-3′)	Size (bp)
GAPDH	Forward: GGCACAGTCAAGGCTGAGAATG	143
	Reverse: ATGGTGGTGAAGACGCCAGTA
Insulin	Forward: AGCGTGGCATTGTGGATCAG	117
	Reverse: TCAAAGGCTTTATTCATTGCAGAGG
Gcg	Forward: ACTGGCTGATTCAAACCAAGATCAC	150
	Reverse: ATGTATTCGTCCGCATGCAAAG
Gck	Forward: AGTATGACCGGATGGTGGATGAA	114
	Reverse: CCAGCTTAAGCAGCACAAGTCGTA
GLUT2	Forward: GACCGGCACATGCTCTCATC	102
	Reverse: TTGGAGCAATCTCGCCAATGTA
Irs1	Forward: AAGCACCTATGCCAGCATCAAC	106
	Reverse: GAGGATTGCTGAGGTCATTTAGGTC
Irs2	Forward: TCTGCCAGCACCTACGCAAG	159
	Reverse: TGAGCAGCGTTGGTTGGAA