Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway

Figure 6

(a) The CAPN2 expression in CRPC tissue and CRPC cells was measured by qRT-PCR and Western blot. (a) CAPN2 expression in CRPC samples was upregulated than that in the nontumor tissues. The median in each triplicate was used to calculate the relative CPAN2 concentration using the comparative method. (b) CAPN2 protein expression in three matched normal/tumor samples was detected by Western blot analysis. GAPDH was used as an internal control. N and T represent normal and tumor tissues, respectively. (c) Immunohistochemistry staining to examine the CAPN2 expression in Pca tissues and adjacent normal tissues (×400 magnification).

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