Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a

Figure 1

The efficiency of miR-29a transfection of scleral fibroblasts and retinal pigment epithelial (RPE) cells. Scleral fibroblasts and RPE cells were grown in six-well plates to 60–70% confluency before transfection. The cells in each well were transfected with 50 nM miR-29a mimics or 50 nM miR-29a inhibitor. The quantitative PCR results showed that, in scleral fibroblasts (a) and RPE cells (b), miR-29a was significantly upregulated by transfection of the miR-29a mimics and downregulated by transfection of the miR-29a inhibitor compared with negative controls (). Note: the error bars show the standard deviations (). The statistical analyses were performed using one-way ANOVA, . NC, negative controls.

(a)

(b)