Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a

Figure 3

Changes in matrix metalloproteinase- (MMP-) 2 mRNA levels in scleral fibroblasts and retinal pigment epithelial (RPE) cells after transfection with miR-29a mimics or inhibitor. Scleral fibroblasts and RPE cells were grown in six-well plates to 60–70% confluency before transfection. The cells in each well of a six-well plate were transfected with 50 nM mimics or 50 nM inhibitor. Quantitative PCR analyses showed that transfection of the miR-29a mimics decreased MMP2 expression in scleral fibroblasts (a) () and RPE cells (b) (), while the inhibitor increased MMP-2 expression in scleral fibroblasts (a) () and RPE cells (b) () 24 hours after transfection compared with negative controls. Note: the error bars show the standard deviations (). The statistical analyses were performed using one-way ANOVA. , . NC, negative control.

(a)

(b)