Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a

Figure 4

Changes in matrix metalloproteinase- (MMP-) 2 protein levels in scleral fibroblasts (a) and retinal pigment epithelial (RPE) cells (b) after transfection with miR-29a mimics or inhibitor. The scleral fibroblasts and RPE cells were grown in six-well plates to 60–70% confluence before transfection. The cells in each well were transfected with 50 nM mimics or 50 nM inhibitor. Western blots showed that the miR-29a mimics decreased MMP2 expression, and the inhibitor increased MMP-2 expression in both scleral fibroblasts and RPE cells 24 hours after transfection. NC, negative control.

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