Regulation of Matrix Metalloproteinase-2 Secretion from Scleral Fibroblasts and Retinal Pigment Epithelial Cells by miR-29a

Figure 5

Changes in matrix metalloproteinase- (MMP-) 2 secretion from scleral fibroblasts and retinal pigment epithelial (RPE) cells after transfection with miR-29a mimics or inhibitor. The protein secreted from scleral fibroblasts and RPE cells was analyzed using enzyme-linked immunosorbent assays (ELISAs). The secretion of MMP-2 by scleral fibroblasts after 48 hours was decreased in cells transfected with the miR-29a mimics compared with negative controls (). The secretion of MMP-2 by scleral fibroblasts after 72 hours was significantly increased in cells transfected with the miR-29a inhibitor compared with negative controls (). The secretion of MMP-2 was higher 72 hours after transfection than 48 hours after transfection in both the miR-29a mimics group () and miR-29a inhibitor group (). (a) The secretion of MMP-2 by RPE cells was significantly decreased after 48 () and 72 () hours in cells transfected with the miR-29a mimics compared with negative controls. The secretion of MMP-2 by RPE cells after 48 hours was significantly increased in cells transfected with the miR-29a inhibitor compared with negative controls (). The secretion of MMP-2 was higher 72 hours after transfection than 48 hours after transfection in both the miR-29a mimics group () and miR-29a inhibitor group () (b). Note: the error bars show the standard deviations (). The statistical analyses were performed using one-way ANOVA for groups at same time point and paired t-tests for between-group analyses at different time points. , . NC, negative control; h, hour.

(a)

(b)