Failure of the PTEN/aPKC/Lgl Axis Primes Formation of Adult Brain Tumours in Drosophila

Figure 1

PTEN mutation activates the PI3K-pAKT pathway in different regions of the larval brain, while increasing aPKC and lowering Lgl at the cell membrane. (a) MARCM PTEN¹¹⁷ LOF clones (GFP⁺) induced in a wild-type background. pAKT staining (red) is positive in the optic lobes (outlined, arrowheads) and in the dorsomedial (DM) region of the brain (arrows). The asterisk marks a PTEN¹¹⁷ mutant clone in the central brain which does not activate AKT. ((b)-(c)) Flp/FRT PTEN¹¹⁷ clones in the OL show aPKC accumulation ((b) white, outlined) and Lgl decrease ((c) white, outlined). (d) Expression pattern of Optix in the larval brain: OL = optic lobe; CB = central brain; NE = neuroepithelium; NB I = type I NBs; NB II = type II NBs; VG = ventral ganglion. Scale bars are 50 μm.

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