Failure of the PTEN/aPKC/Lgl Axis Primes Formation of Adult Brain Tumours in Drosophila

Figure 2

The activated form of aPKC induces hyperplastic growth of the larval NE. ((a)–(c)) Representative brains from Optix- late L3 larvae grown at 25°C. (a) Surface section showing aPKC (white) membrane enrichment (middle panel) and dIAP expression (lower panel) in the Optix NE domain (GFP⁺, upper panel). (b) Cross-section showing the multilayered structure of the NE and dIAP expression (lower panel) within the Optix domain (GFP⁺, upper panel). (c) Surface section displaying Lgl (white) release from the membrane (middle panel) and MYC expression (lower panel) within the Optix domain (GFP⁺). Green arrowheads indicate the boundaries between and wild-type OL, and yellow arrowheads indicate the boundaries between and wild-type NE. Scale bars are 50 μm.

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