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BioMed Research International
Volume 2017, Article ID 2747215, 10 pages
https://doi.org/10.1155/2017/2747215
Research Article

Different Cellular Response of Human Mesothelial Cell MeT-5A to Short-Term and Long-Term Multiwalled Carbon Nanotubes Exposure

1Zhejiang Academy of Medical Sciences, Hangzhou, China
2Jiading District Center for Disease Control and Prevention, Shanghai, China
3Hangzhou Normal University School of Public Health, Hangzhou, China

Correspondence should be addressed to Lijin Zhu; nc.moc.smajz@uhznijil and Jun Yang; nc.ude.ujz@etatsag

Received 10 April 2017; Revised 15 June 2017; Accepted 5 July 2017; Published 8 August 2017

Academic Editor: Brad Upham

Copyright © 2017 Li Ju et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Despite being a commercially important product, multiwalled carbon nanotubes (MWCNTs) continue to raise concerns over human health due to their structural similarity to asbestos. Indeed, exposure to MWCNT has been shown to induce lung cancer and even mesothelioma, but contradictory results also exist. To clarify the potentially carcinogenic effects of rigid and rod-like MWCNT and to elucidate the underlying mechanisms, the effects of MWCNT on human mesothelial cell MeT-5A were examined throughout 3 months of continuous exposure, including cytotoxicity, genotoxicity, and cell motility. It was found that MWCNT did not affect MeT-5A cell proliferation at 10 μg/cm2 within 72 h treatment, but under the same condition, MWCNT induced genotoxicity and perturbed cell motility. In addition, MeT-5A cells demonstrated different cellular responses to MWCNT after short-term and long-term exposure. Taken together, our results indicated a possible carcinogenic potential for MWCNT after long-term treatment, in which Annexin family proteins might be involved.