OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

Figure 3

Expression of OCT4B1 in DPCs with lentiviral transfection. Green fluorescence was observed in the nucleus and cytoplasm of both pCDH-OCT4B1-flag and pCDH-flag cells. Red fluorescence representing flag octa peptide was expressed only in the nucleus and cytoplasm of pCDH-OCT4B1-flag-GFP cells. (a) (A1–A4) No green or red fluorescence was expressed in nontransfected cells (×400). (b) (B1–B4) Blue and green fluorescence were expressed in pCDH-GFP cells (×400). (c) (C1–C4) Blue, green, and red fluorescence could be observed in the OCT4B1 overexpression cells (×400). (d) Western blot showed that no protein band was detected in nontransfected pCDH-GFP cells. 23 KD (OCT4B1) and 33 KD (OCT4B) protein bands were detected with flag antibody. (e) There was a blurred band of 33 KD detected with OCT4B antibody, demonstrating that the expression of OCT4B was increased after OCT4B1 transfection.

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