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BioMed Research International
Volume 2017 (2017), Article ID 2756891, 10 pages
https://doi.org/10.1155/2017/2756891
Research Article

OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

1Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China
2Institute of Health and Biomedical Innovation and Science and Engineering Faculty, Queensland University of Technology, Brisbane, QLD, Australia

Correspondence should be addressed to Xi Wei

Received 8 January 2017; Revised 11 March 2017; Accepted 16 March 2017; Published 4 April 2017

Academic Editor: Takashi Yazawa

Copyright © 2017 Lu Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. D. Tziafas, A. J. Smith, and H. Lesot, “Designing new treatment strategies in vital pulp therapy,” Journal of Dentistry, vol. 28, no. 2, pp. 77–92, 2000. View at Publisher · View at Google Scholar · View at Scopus
  2. N. Tamaoki, K. Takahashi, T. Tanaka et al., “Dental pulp cells for induced pluripotent stem cell banking,” Journal of Dental Research, vol. 89, no. 8, pp. 773–778, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. X. Wei, J. Ling, L. Wu, L. Liu, and Y. Xiao, “Expression of mineralization markers in dental pulp cells,” Journal of Endodontics, vol. 33, no. 6, pp. 703–708, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. M. V. Thomas and D. A. Puleo, “Infection, inflammation, and bone regeneration: a paradoxical relationship,” Journal of Dental Research, vol. 90, no. 9, pp. 1052–1061, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. R. C. Jacinto, B. P. F. A. Gomes, H. N. Shah, C. C. Ferraz, A. A. Zaia, and F. J. Souza-Filho, “Quantification of endotoxins in necrotic root canals from symptomatic and asymptomatic teeth,” Journal of Medical Microbiology, vol. 54, no. 8, pp. 777–783, 2005. View at Publisher · View at Google Scholar · View at Scopus
  6. C. Kitamura, T. Nishihara, Y. Ueno et al., “Effects of sequential exposure to lipopolysaccharide and heat stress on dental pulp cells,” Journal of Cellular Biochemistry, vol. 99, no. 3, pp. 797–806, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. H. Yang, Y. T. Zhu, R. Cheng et al., “Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro,” Brazilian Journal of Medical and Biological Research, vol. 43, no. 11, pp. 1027–1033, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. S. J. Fokkema, “Peripheral blood monocyte responses in periodontitis,” International Journal of Dental Hygiene, vol. 10, no. 3, pp. 229–235, 2012. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Geng, S. Xiao, Z. Zheng, S. Song, and L. Zhang, “Gambogic acid protects from endotoxin shock by suppressing pro-inflammatory factors in vivo and in vitro,” Inflammation Research, vol. 62, no. 2, pp. 165–172, 2013. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Yokoyama, T. Ukai, E. R. Ayon Haro, T. Kishimoto, Y. Yoshinaga, and Y. Hara, “Membrane-bound CD40 ligand on T cells from mice injected with lipopolysaccharide accelerates lipopolysaccharide-induced osteoclastogenesis,” Journal of Periodontal Research, vol. 46, no. 4, pp. 464–474, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. H. R. Schöler, S. Ruppert, N. Suzuki, K. Chowdhury, and P. Gruss, “New type of POU domain in germ line-specific protein Oct-4,” Nature, vol. 344, no. 6265, pp. 435–439, 1990. View at Publisher · View at Google Scholar · View at Scopus
  12. M. Pesce and H. R. Schöler, “Oct-4: control of totipotency and germline determination,” Molecular Reproduction and Development, vol. 55, no. 4, pp. 452–457, 2000. View at Publisher · View at Google Scholar · View at Scopus
  13. B. R. Krishnan, I. Jamry, and D. D. Chaplin, “Feature mapping of the HLA class I region: localization of the POU5F1 and TCF19 genes,” Genomics, vol. 30, no. 1, pp. 53–58, 1995. View at Publisher · View at Google Scholar · View at Scopus
  14. S. I. Papamichos, V. Kotoula, B. C. Tarlatzis, T. Agorastos, K. Papazisis, and A. F. Lambropoulos, “OCT4B1 isoform: the novel OCT4 alternative spliced variant as a putative marker of stemness,” Molecular Human Reproduction, vol. 15, no. 5, pp. 269–270, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. M. A. Rijlaarsdam, H. A. D. M. Van Herk, A. J. M. Gillis et al., “Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours,” British Journal of Cancer, vol. 105, no. 6, pp. 854–863, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. M. H. Asadi, S. J. Mowla, F. Fathi, A. Aleyasin, J. Asadzadeh, and Y. Atlasi, “OCT4B1, a novel spliced variant of OCT4, is highly expressed in gastric cancer and acts as an antiapoptotic factor,” International Journal of Cancer, vol. 128, no. 11, pp. 2645–2652, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Gazouli, M. G. Roubelakis, G. E. Theodoropoulos et al., “OCT4 spliced variant OCT4B1 is expressed in human colorectal cancer,” Molecular Carcinogenesis, vol. 51, no. 2, pp. 165–173, 2012. View at Publisher · View at Google Scholar · View at Scopus
  18. Y. Gao, X. Wang, J. Han et al., “The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B,” Journal of Genetics and Genomics, vol. 37, no. 7, pp. 461–465, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. E. Farashahi Yazd, M. R. Rafiee, M. Soleimani, M. Tavallaei, M. K. Salmani, and S. J. Mowla, “OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response,” Cancer Letters, vol. 309, no. 2, pp. 170–175, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Asadzadeh, M. H. Asadi, N. Shakhssalim et al., “A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors,” Urology Journal, vol. 9, no. 3, pp. 574–580, 2012. View at Google Scholar · View at Scopus
  21. L. Liu, X. Wei, J. Ling, L. Wu, and Y. Xiao, “Expression pattern of Oct-4, sox2, and c-Myc in the primary culture of human dental pulp derived cells,” Journal of Endodontics, vol. 37, no. 4, pp. 466–472, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. J. Wang, X. Wei, J. Ling, Y. Huang, Y. Huo, and Y. Zhou, “The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells,” Biochemical and Biophysical Research Communications, vol. 400, no. 3, pp. 334–339, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. G. T.-J. Huang, W. Sonoyama, J. Chen, and S. H. Park, “In vitro characterization of human dental pulp cells: various isolation methods and culturing environments,” Cell and Tissue Research, vol. 324, no. 2, pp. 225–236, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Takahashi and S. Yamanaka, “Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors,” Cell, vol. 126, no. 4, pp. 663–676, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. Y. Li, Q. Zhang, X. Yin et al., “Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules,” Cell Research, vol. 21, no. 1, pp. 196–204, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. J. Nichols, B. Zevnik, K. Anastassiadis et al., “Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4,” Cell, vol. 95, no. 3, pp. 379–391, 1998. View at Publisher · View at Google Scholar · View at Scopus
  27. J. Kehler, E. Tolkunova, B. Koschorz et al., “Oct4 is required for primordial germ cell survival,” EMBO Reports, vol. 5, no. 11, pp. 1078–1083, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. M. R. Mirzaei, A. Najafi, M. K. Arababadi, M. H. Asadi, and S. J. Mowla, “Altered expression of apoptotic genes in response to OCT4B1 suppression in human tumor cell lines,” Tumor Biology, vol. 35, no. 10, pp. 9999–10009, 2014. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Maragkoudaki, A. Vaiopoulou, G. E. Theodoropoulos et al., “Specific detection of OCT4 isoforms in inflammatory bowel disease,” Gut Pathogens, vol. 7, article 25, 2015. View at Publisher · View at Google Scholar · View at Scopus
  30. L. Liu, X. Wei, R. Huang, J. Ling, L. Wu, and Y. Xiao, “Effect of bone morphogenetic protein-4 on the expression of Sox2, Oct-4, and c-Myc in human periodontal ligament cells during long-term culture,” Stem Cells and Development, vol. 22, no. 11, pp. 1670–1677, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. Y. Atlasi, S. J. Mowla, S. A. M. Ziaee, P. J. Gokhale, and P. W. Andrews, “OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells,” STEM CELLS, vol. 26, no. 12, pp. 3068–3074, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. G. Cauffman, H. Van de Velde, I. Liebaers, and A. Van Steirteghem, “Oct-4 mRNA and protein expression during human preimplantation development,” Molecular Human Reproduction, vol. 11, no. 3, pp. 173–181, 2005. View at Publisher · View at Google Scholar · View at Scopus
  33. G. Cauffman, I. Liebaers, A. Van Steirteghem, and H. Van De Velde, “POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos,” Stem Cells, vol. 24, no. 12, pp. 2685–2691, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. X. Wang, Y. Zhao, Z. Xiao et al., “Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response,” Stem Cells, vol. 27, no. 6, pp. 1265–1275, 2009. View at Publisher · View at Google Scholar · View at Scopus
  35. M. R. Hunter, N. L. Grimsey, and M. Glass, “Sulfation of the FLAG epitope is affected by co-expression of G protein-coupled receptors in a mammalian cell model,” Scientific Reports, vol. 6, article 27316, 2016. View at Publisher · View at Google Scholar
  36. A. Einhauer and A. Jungbauer, “The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins,” Journal of Biochemical and Biophysical Methods, vol. 49, no. 1–3, pp. 455–465, 2001. View at Publisher · View at Google Scholar