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BioMed Research International
Volume 2017 (2017), Article ID 2794040, 18 pages
https://doi.org/10.1155/2017/2794040
Research Article

The Effect of Different Pollination on the Expression of Dangshan Su Pear MicroRNA

1School of Life Science, Anhui Agricultural University, Changjiang West Road, No. 130, Hefei 230036, China
2Horticultural Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China

Correspondence should be addressed to Yongping Cai

Received 3 July 2016; Revised 18 November 2016; Accepted 11 January 2017; Published 10 April 2017

Academic Editor: Limei Qiu

Copyright © 2017 Xi Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The high-throughput sequencing of pear “Dangshan Su” × “Yali” (whose fruits lignin and stone cell content are high and quality is poor) and pear “Dangshan Su” × “Wonhwang” (whose fruits with low content of lignin and stone cell and the quality are better ) found that the expressions of these two miRNAs (pyr-1809 and pyr-novel-miR-144-3p) were significantly different; their corresponding target genes encode two kinds of laccase (Pbr018935.1 and Pbr003857.1). qRT-PCR results showed that these two enzymes are involved in the formation of lignin and stone cells and the existence of these two miRNAs has a negative effect on them. It was concluded that the effect of pollination on the development of stone cells may affect the synthesis of lignin, through the regulation of laccase controlled by miRNAs, and ultimately affect the formation of stone cell and fruit quality.