His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?

Figure 1

-FLAG tag initial geometries. The globule of the hIFNγ homodimer is depicted in grey ribbons and its molecular surface, in white bubbles. The binding interfaces for each of the two receptor subunits are shown in yellow bubbles. The previously reconstructed C-termini are in blue. The added N-terminal peptides are in green for conformation G1 and in red for conformation G2.