His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?

Figure 7

Summary of the trajectories. The conformations of the N-terminal peptides are shown in red and of the C-termini, in blue. The globule of hIFNγ is in grey ribbon and the receptor binding sites, in yellow bubbles. In the simulations starting from 1 (a), the added peptides remain predominantly close to the globule and the binding sites in particular. In the 2 simulation (b), one of the N-terminal peptides remains fairly distant to the globule and interacts loosely with the respective C-terminus, whereas the peptide in the other monomer approaches the globule.

(a)

(b)