Research Article
His-FLAG Tag as a Fusion Partner of Glycosylated Human Interferon-Gamma and Its Mutant: Gain or Loss?
Figure 8
SDS-PAGE of hIFNγ after -FLAG tag cleavage and IMAC chromatography. Lane 1: fraction before digestion; Lane 2: eluted fraction containing cleaved hIFNγ; Lane M: protein molecular weight markers in kDa. The arrow indicates the position of the cleaved hIFNγ.