Purification and Characterization of an ATPase GsiA from Salmonella enterica

Figure 4

In vivo analysis of GsiA interaction with other components of glutathione importer. The genes were cloned into pET11a-link-NGFP and pMRBAD-link-CGFP which were referred to as pN and pC. pN-Z and pC-Z were positive control plasmids. BL21(DE3) transformants were induced with 10 μM IPTG and 0.2% arabinose. The plates were incubated at 20°C for 2 days. If proteins could interact with each other, the cell showed reproducible green fluorescence under UV light. Numbers 1 to 10 were cells transformed with pN-gsiA and pC-gsiA; pN-gsiA and pC-gsiB; pN-gsiA and pC-gsiC; pN-gsiA and pC-gsiD; pN-gsiC and pC-gsiD; pN-gsiC and pC-gsiA; pN-gsiD and pC-gsiA; pN-Z and pC-Z; pN-gsiA and pC-Z; pN-Z.