Purification and Characterization of an ATPase GsiA from Salmonella enterica

Table 1

Primers for gsiA expression, deletion, and protein interactions.

Primer	Sequence 5′-3′
gsiA del-F	TGGGGATATGCCGGGGATACGCCGACCACAGGAATTTACCG; GGAATAAGGAGCCGCAGGTGTAGGCTGGAGCTGCTTC
gsiA del-R	CATTTATGCGTAATAAATTGCGTCATGTTGTTCTCCTGAAACC; CTGCCTGTCTGAATGCATATGAATATCCTCCTTAG
ggt del-F	CGTTGTGGGCCAGGATTAAACGGGACATACACCCATTTATCT; GGAGAAAAACAACGGTGTAGGCTGGAGCTGCTTC
ggt del-R	TACTCACGGCATAATCTGCAAATAGCCCGGTGTCTGACAC-; CGGGCCTGGAATTAAAAACATATGAATATCCTCCTTAG
ggt-F	ATGAAACCAACGTTTATGCGC
ggt-R	TCAGTATCCCGCCGTTAAATC
gsiA-F1	CATG CCATGG CA ATGCCGCACAGCGATGAAC
gsiA-R1	CCC AAGC TTATAAGCGCGAGAGCGCATT
gsiA-F2	CCGCTCGAG ATGCCGCACAGCGATGAAC
gsiA-R2	TCA CCCGGGTTATAAGCGCGAGAGCGCAT
gsiA-F3	CATGCCATGGCA ATGCCGCACAGCGATGAAC
gsiA-R3	CATG GACGTC TAAGCGCGAGAGCGCATTATC
gsiB-F	CATGCCATGGCA ATGACGCAATTTATTACGCATAAAT
gsiB-R	CATG GACGTC CTTCAAATCCGCATCGTCAAAG
gsiC-F1	CCGCTCGAG ATGCTTAACTATGTTCTCAAGCGC
gsiC-R1	CGCGGATCC TTACTTATACCTGATAGCCGGATTA
gsiC-F2	CATGCCATGGCA ATGCTTAACTATGTTCTCAAGCGC
gsiC-R2	CATG GCATGCCTTATACCTGATAGCCGGATTAATG
gsiD-F1	CCGCTCGAGATGCGATTATTTAACTGGCGC
gsiD-R1	CGCGGATCCTTACCCCTTAATCTTCGGGTCC
gsiD-F2	CATGCCATGGCA ATGCGATTATTTAACTGGCGC
gsiD-R2	CATG GACGTC CCCCTTAATCTTCGGGTCCAG