MicroRNA-16-5p Inhibits Osteoclastogenesis in Giant Cell Tumor of Bone

Figure 2

miR-16-5p inhibits osteoclast formation. (a) Quantification of miR-16-5p mRNA expression during RANKL-induced osteoclastogenesis. (b–d) TRAP staining showing a decrease in the number of osteoclasts following treatment with the miR-16-5p mimic. (e–g) TRAP staining showing an increase in the number of osteoclasts after treatment with the miR-16-5p inhibitor. Mimic represents the agomir for miR-16-5p, inhibitor represents the antagomir for miR-16-5p, mimic control represents the negative control for miR-16-5p mimic, and inhibitor control represents the negative control for miR-16-5p inhibitor. Scale bars represent 10 μm. . P values were analyzed using the one-way ANOVA test. All data are representative of three independent experiments.