Platinum-Based Drugs Differentially Affect the Ultrastructure of Breast Cancer Cell Types

Figure 3

TEM micrographs of PDBs effect on MCF-7 cell line. (a) illustrates the effect of PBDs on the intracellular organelles of MCF-7 cells. PBDs deposits (D) were scattered over the intercellular organelles (N; nucleus, Nu; nucleolus, F; fat droplet, M; mitochondria, ER; endoplasmic reticulum, G; Golgi apparatus, P; phagosome, and V; villi) causing structural changes. Micrographs are screened at scale of 1–10 μM. (b) MCF-7 treated cells with PBDs compared to control. (A) illustrates the ultrastructural change of mitochondria (arrows), (A)(i) untreated (20000x), (A)(ii) 10 μM Cisplatin treated cell for 2 h (25000x), (A)(iii) 10 μM Carboplatin treated cells for 4 h (20000x), and (A)(iv) 20 μM Oxaliplatin treated cell for 4 h (25000x). (B) illustrates chromatin condensation and fragmentation (arrows), (B)(i) untreated (4000x), (B)(ii) 10 μM Cisplatin treated cells for 4 h (5000x), (B)(iii) 20 μM Carboplatin treated cells for 2 h (12000x), and (B)(iv) 20 μM Oxaliplatin treated cell for 2 h (5000x). (C) demonstrates phagosome formation (arrows), (C)(i) untreated (12000x), (C)(ii) 20 μM Cisplatin treated cells for 2 h (12000x), (C)(iii) 10 μM Carboplatin treated cells for 2 h (8000x), (C)(iv) 10 μM Oxaliplatin treated cell for 4 h (10000x). Micrographs are screened at scale of 1–10 μM. (c) MCF-7 treated cells with 20 μM of Oxaliplatin for 4 h showing specific characteristics of nucleolus (cap appearance). (d) MCF-7 treated cells with Oxaliplatin demonstrating specific feature of fat droplet (arrow).

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