Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis

Figure 5

Histological analysis of mice liver, spleen, and kidney. Hematoxylin-eosin staining. The scale indicated as 20 μm. (a) to (c) were histological sections of mice liver, (d) to (f) were mice spleen, and (g) to (i) were mice kidney. (a), (d), and (g) were the tissues from control group; (b), (e), and (h) were the tissues from PBS + E. coli group; (c), (f), and (i) were from F17A-MF59 + E. coli group. (b) Hepatocyte degeneration with cellular swelling and dissolution and disappearance of nucleus; structure disturbance of hepatic cords; Kupffer cells increased and lymphocyte aggregation (arrows) occurred. (c) Swelling of hepatocytes and dissolution and disappearance of nucleus; structural abnormality of hepatic cords; Kupffer cells (arrows) increased. (e) Serious hyperaemia and megakaryocytes (arrows) increased in splenic red pulps. (f) Megakaryocytes (arrows) increased in splenic red pulps. (h) Hyperaemia in the glomeruli capillaries, congestion (arrows) in renal tubular interstitium, degeneration of renal tubular epithelial cells presenting swelling, karyopyknosis, and karyolysis, and reddish material (arrows) exuded in lumen renal tubulars. (i) Mild hyperaemia in the glomeruli capillaries, mild congestion (arrows) in renal tubular interstitium, and local karyopyknosis (arrows) of renal tubular epithelial cells.