Research Article
Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis
Table 1
Treatment in animal experiments.
| | Experiment | Groups | Treatment | Time | | Inoculum | E. coli challenge |
| | Safety assay | C:
V: | 400 μL/mouse | None | 21 days |
| | Immunogenicity | C:
F:
V: | 200 μL/mouse | None | 8 weeks |
| | Survival test | C:
V: | 200 μL/mouse | 4 × 108 CFU,
6 weeks after inoculation | 21 days |
| | Bacteria clearance test | C:
V: | 200 μL/mouse | 8 × 107 CFU,
6 weeks after inoculation | 7 days |
| | Histopathology | C:
V: | 200 μL/mouse | 8 × 107 CFU,
6 weeks after inoculation | 9 days |
| | Classification of T lymphocytes | C:
V: | 200 μL/mouse | 8 × 107 CFU,
6 weeks after inoculation | 72 hours |
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C: control group treated with PBS; V: vaccinated group; F: treated only with F17A.
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