Reduction of Mosquito Survival in Mice Vaccinated with Anopheles stephensi Glucose Transporter

Figure 1

Molecular Phylogenetic Analysis by Maximum Likelihood Method. Using the Maximum Likelihood method based on the Le_Gascuel_2008 model [20], the tree with the highest log likelihood (−3359.935) is shown. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 1.65)). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 5 amino acid sequences with respective VectorBase accession number: AGAP007752: Anopheles gambiae, ASTE006385: A. stephensi, ASIS016443: A. sinensis, ADAC010569: A. darlingi, and CG15406: Drosophila melanogaster (outgroup). All positions containing gaps and missing data were eliminated and bootstrap values for internal branches are shown. Evolutionary analyses were conducted in MEGA6 [21].