XBP1-Mediated BiP/GRP78 Upregulation Copes with Oxidative Stress in Mosquito Cells during Dengue 2 Virus Infection

Figure 7

Effect of BiP/GRP78 on the synthesis of the viral envelope (E) protein in C6/36 cells. (a) A microRNA-based BiP/GRP78 knockdown system (miBiP) was constructed; the efficient reduction of BiP/GRP78 expressed by C6/36 cells was seen. (b) Viral RNA expression had increased up to 2.5-fold by 24 hpi in C6/36 cells with BiP/GRP78 knockdown. (c) A lower level of viral E protein was detected in dengue 2 virus- (DENV2-) infected C6/36 cells (24 h postinfection (hpi)) with BiP/GRP78 knockdown compared to cells without knockdown. Results indicated that BiP/GRP78 was associated with viral E protein synthesis but had no effect on viral RNA replication.

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