In Vivo Murine Model of Leukemia Cell-Induced Spinal Bone Destruction

Figure 1

Constitutively active STAT5 signaling promotes malignant transformation in myeloid leukemia cells, showing enhanced colony-forming ability and increased proliferative capacity in vitro and in vivo. Stable HL-60 leukemia cells with enforced expression of CA-STAT5 were generated by retrovirus infection. (a) Western blot analysis of the expression of STAT5A, pY-STAT5a, and c-Myc. The cell extracts were probed with antibodies against STAT5A, pY-STAT5a, c-Myc, and GAPDH (loading control) as indicated. (b, c) The qPCR analyses of the expression of STAT5A and c-Myc in the stable HL-60 cells. (d) Proliferation of the stable HL-60 cells evaluated by the trypan blue exclusion method. HL-60/MIG-GFP and HL-60/MIG-STAT5CA-GFP cells were analyzed using a colony formation assay. Morphological image (e), colony size (f), and rate of colony formation (g) are shown. The HL-60/GFP and HL-60/CA-STAT5 cells were resuspended and injected subcutaneously into both sides of the backs of male nude mice (BALB/C, nu/nu) to establish a human leukemia xenograft model. (h) Representative tumors removed from mice are shown. The results are shown as the mean values ± SD of 3 independent experiments. , , compared to the control.

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