(a)
(b)
(c)
(d)
Figure 1: Proliferation of primary osteoblasts isolated from osteoporotic patients was inhibited. (a) Optical microscopic observation of osteoblasts from normal and osteoporotic subjects after 72 h incubation. (b) Proliferation of osteoblasts from osteoporotic patients was inhibited. Osteoporotic and normal osteoblasts were incubated for 72 h. The cell viability at different time points was determined by MTT assay. (c) The EDU stain also performed in cells treated as described above with a magnification of 200. (d) Osteoporotic osteoblasts exhibited higher apoptosis level. Apoptosis was determined by Annexin V/PI assay. compared with normal group.