Recombinant Vaccinia Viruses Coding Transgenes of Apoptosis-Inducing Proteins Enhance Apoptosis But Not Immunogenicity of Infected Tumor Cells

Figure 5

Ecto-CRT analysis. MDA-MB-231 and MX-7 cells were treated with VVs (0.05 and 0.5 PFU/cell) for the indicated time; RLS were treated for 24 h. Control cells were treated with PBS. Cells were stained with anti-CRT antibody, followed by immunofluorescence detection of CRT exposure. (a) CRT exposure after VVs treatment. Representative data of three independent experiments. Cells were treated with VVs (0.5 PFU/cell) for 24 h. Isotype control panel is presented for cells treated with PBS. (b) The results represent the % of CRT-positive cells ± SD.