BioMed Research International / 2017 / Article / Fig 6

Research Article

Recombinant Vaccinia Viruses Coding Transgenes of Apoptosis-Inducing Proteins Enhance Apoptosis But Not Immunogenicity of Infected Tumor Cells

Figure 6

Recombinant VVs induce immunogenic cell death in MDA-MB-231 cancer cells. Control cells were treated with PBS. (a) Western blot analysis of HMGB1 expression in cell lysates 3, 24, and 48 h after infection (0.05 PFU/cell). One representative of three independent experiments is shown. (b) Relative amount of extracellular ATP, measured in conditioned media of virus-treated cells (0.05 PFU/cell). RLU: relative luminescent unit; (c) analysis of surface-exposed HSP70 by flow cytometry. Cells were stained 24 h after infection. versus control (nontreated cells).
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