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BioMed Research International
Volume 2017 (2017), Article ID 3634915, 8 pages
https://doi.org/10.1155/2017/3634915
Research Article

Chemopreventive Potential of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats

1Department of Medical Education and Research and Department of Pharmacy, Yuan’s General Hospital, Kaohsiung, Taiwan
2Institute of Clinical Dentistry and Department of Dentistry, National Taiwan University Hospital and National Taiwan University Medical College, Taipei, Taiwan
3Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, Taiwan
4Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan
5Department of Health-Business Administration, School of Nursing, Fooyin University, Kaohsiung, Taiwan
6Department of Radiology and Nuclear Medicine, Yuan’s General Hospital, Kaohsiung, Taiwan
7Department of Nutrition and Health Science, School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan
8Department of Pharmacology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Correspondence should be addressed to Chung-Yi Chen; wt.ude.yf@773xx and Chien-Hsing Lee; moc.liamg@8180eelhc

Received 5 June 2017; Accepted 2 October 2017; Published 7 November 2017

Academic Editor: Koichiro Wada

Copyright © 2017 Chien-Liang Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) has been shown to have antioxidative and anti-inflammatory effects. Oxidative and inflammatory reactions are related to the development of colorectal carcinoma (CRC). In the present study, we characterized the preventive activities of THSG on colon carcinogenesis using the azoxymethane- (AOM-) mediated rat colon carcinogenesis model. F344 male rats were randomly divided into 5 groups (untreated and AOM model rats treated with or without THSG at 30, 150, or 250 mg/kg) after which the numbers of aberrant crypt foci (ACF) were assessed in the colon tissues of all rats. The expressions of nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), matrix metalloproteinase proteins (MMPs), and carcinoembryonic antigen (CEA) were measured as effective early predictors of CRC using western blot analysis. Treatment with THSG (150 or 250 mg/kg) induced a 50% reduction in total colonic ACF formation (). Furthermore, our results revealed a downregulation of CEA and NF-κB protein levels in the reduced number of ACF elicited by treatment with THSG, whereas levels of COX-2 and MMPs proteins were not changed. Collectively, THSG may be a promising natural lead compound or drug candidate for treating early phases of CRC.