A Study of IL-1β, MMP-3, TGF-β1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population

Table 1

PCR primers and single base pair (bp) extension primers of 4 SNPs at IL-1β, GDF5, MMP-3, and TGF-β1.

Genes	SNPs	PCR primer sequence (5′→3′)	Single bp extension primer sequence
IL-1β	rs1143627-F	ACGTTGGATGTTGTGCCTCGAAGAGGTTTG	TCCCTCGCTGTTTTTAT
	rs1143627-R	ACGTTGGATGTCTCAGCCTCCTACTTCTGC
MMP-3	rs650108-F	ACGTTGGATGTCAGGTAGAGGTGACAAGTG	CCAGTGGGTGAGGTTAGA
	rs650108-R	ACGTTGGATGGTCACTGTCTCATTGTGTGT
TGF-β1	rs1800469-F	ACGTTGGATGTACAGGTGTCTGCCTCCTGA	ACTCCTGACCCTTCCATCC
	rs1800469-R	ACGTTGGATGAAGAGGGTCTGTCAACATGG
GDF5	rs143383-F	ACGTTGGATGCAGCAGCTGAAAATAACTCG	TCTTGAAAGGAGAAAGCC
	rs143383-R	ACGTTGGATGTTCAAGCCCTCAGTCAGTTG

PCR: polymerase chain reaction; SNP: single nucleotide polymorphism.