PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells

Figure 3

Inhibiting PTEN could promote RPE cells growth but had no impacts on cell apoptosis. RPE cells were stably transfected with PTEN shRNA plasmids. (a) The knockdown efficiency of PTEN was measured by Real-Time PCR. (b) Western blots were conducted to detect the PTEN protein expression level and use α-Tubulin as an internal control. (c) Bar graphs represent the relative PTEN protein levels. (d) Apoptosis of cells was quantified by Multicaspase/7-AAD stain and analyzed by flow cytometry. (e) Cell viability was detected by MTT assay. The results are expressed as mean ± SEM from at least 3 experiments (, , t test, , bars represent SD).

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