PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells

Figure 4

Inhibiting PTEN enhanced levels of UVB-induced apoptosis and activation of p53 in RPE cells. The vector and inhibiting PTEN cells were irradiated with UVB (60 mJ/cm²) and then incubated for 24 h. “v” represented vector; “sh-4 and sh-5” represented sh-PTEN4 and sh-PTEN5. (a) The relative mRNA expression level of PTEN was determined in RPE cells by Real-Time PCR. The result was normalized to GAPDH. (b) The expression levels of proteins were analyzed by western blot. (c) Apoptosis of cells was measured by flow cytometry. (d) Data are represented as the mean ± SD of three independent experiments (, t test).

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