PTEN Reduced UVB-Mediated Apoptosis in Retinal Pigment Epithelium Cells

Figure 6

p53 inhibitor decreased UVB-induced apoptosis. RPE cells were pretreated with p53 inhibitor Pifithrin-α (10 μM) for 1 h and then incubated for 24 h. (a) Cells were stained with Multicaspase/7-AAD for quantitative measurement using flow cytometry. (b) Western blots were conducted to detect p53 protein expression level and use α-Tubulin as an internal control. RPE cells were pretreated with p53 inhibitor Pifithrin-α (10 μM) for 1 h and these cells were exposed to UVB (60 mJ/cm²) and then incubated for 24 h. “v” represented vector and “sh-4 and sh-5” represented sh-PTEN4 and sh-PTEN5. (c) Cells were stained with Multicaspase/7-AAD for quantitative measurement using flow cytometry. (d) A representative apoptosis along with the statistical data was shown. (e) Western blots were conducted to detect expression levels of protein and use α-Tubulin as a loading control. (f) Using Image J quantized the ratio of p53. These data are shown as the mean ± SD of three independent experiments (, , t test).

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